Application of micellar electrokinetic capillary chromatography for quantitative analysis of quercetin in plant materials.

نویسندگان

  • E Dadáková
  • E Procházková
  • M Krízek
چکیده

A simple method for the routine determination of quercetin in biological samples was developed. The method consists of hydrolysis of bonded quercetin, its isolation and preconcentration on solid-phase extraction (SPE) column and a final analytical step using micellar electrokinetic capillary chromatography. The working range, linear range, the limit of quantification, and the measurement uncertainty were tested in validation. The method is suitable for quercetin determination in fruit and vegetable samples.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Principles of micellar electrokinetic capillary chromatography applied in pharmaceutical analysis.

Since its introduction capillary electrophoresis has shown great potential in areas where electrophoretic techniques have rarely been used before, including here the analysis of pharmaceutical substances. The large majority of pharmaceutical substances are neutral from electrophoretic point of view, consequently separations by the classic capillary zone electrophoresis; where separation is base...

متن کامل

Evaluation of variation of acteoside and three major flavonoids in wild and cultivated Scutellaria baicalensis roots by micellar electrokinetic chromatography.

Micellar electrokinetic chromatography (MEKC) conditions were developed to analyze the constituents of Scutellariae Radix (SR) and Scutellaria baicalensis roots. Using the MEKC method, the major flavonoid constituents of baicalin, baicalein and wogonin of wild and cultivated S. baicalensis roots were compared. In a preliminary comparison of electropherogram, one special peak was found in a wild...

متن کامل

Application of Capillary Electrophoresis to Analysis of Tricyclic Psychotropic Drugs

Two separation modes of capillary electrophoresis (CE): capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MECC) were used for toxicological analysis of blood samples and pharmaceutical preparations. These two techniques were tested on commonly used phenothiazines (PHE) and tricyclic antidepressants (TCA), which are drugs of forensic interest. The analyti...

متن کامل

Determination of imidacloprid and its metabolite 6-chloronicotinic acid in greenhouse air by application of micellar electrokinetic capillary chromatography with solid-phase extraction.

A method is described for the analysis of the insecticide imidacloprid [1-(6-chloro-3-pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine] and its metabolite 6-chloronicotinic acid by micellar electrokinetic chromatography with diode-array detection at 270 and 227 nm, respectively. The best results were obtained using sodium dodecyl sulphate at a concentration of 60 mM and a running buffer of NH4...

متن کامل

Fast determination of Sudan dyes in chilli tomato sauces using partial filling micellar electrokinetic chromatography.

A new method based on partial filling micellar electrokinetic chromatography (MEKC) for the quantitative determination of Sudan dyes (I, II, III, and IV) in chilli sauces is presented. The separation is achieved filling 25% of the capillary with a MEKC buffer composed of 40 mM NH(4)HCO(3), 25 mM sodium dodecyl sulfate, and 32.5% (v/v) acetonitrile (ACN). The rest of the capillary is filled usin...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Electrophoresis

دوره 22 8  شماره 

صفحات  -

تاریخ انتشار 2001